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Manual of Practical Biochemistry, 3rd Edition
M D Rafi
Price
350.00
ISBN
9789389211658
Language
English
Pages
296
Format
Paperback
Dimensions
180 x 240 mm
Year of Publishing
2020
Territorial Rights
World
Imprint
Universities Press

This book introduces some of the most widely used experimental procedures in biochemistry for students of MBBS and other allied sciences. In this new edition, all the chapters have been thoroughly revised to conform to the Competency-Based Medical Education (CBME) curriculum.

Salient features

  • A wide range of qualitative analysis and quantitative estimations and measurements in biochemistry explained with emphasis on principles, procedures, observations and clinical interpretations
  • Theory of the underlying principles and procedures included so that students are able to appreciate the biochemical basis of the demonstrated experiments/techniques
  • Covers all the competencies prescribed in the CBME curriculum
  • Several new topics or expanded treatment of topics such as electrophoresis, chromatography, estimation of pH and preparation of buffers, biomedical waste management, isolation and quantification of DNA, and biochemical tests for common clinical conditions included in this edition
  • Features viva questions commonly encountered in practical examinations
  • Includes MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies to aid students in their preparations for competitive examinations

Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is well-known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.

Preface to the Third Edition
Preface to the First Edition

QUALITATIVE EXPERIMENTS

1. Safety in the Laboratory

2. Reactions of Carbohydrates

Reactions of Glucose
Reactions of Fructose
Reactions of Maltose
Reactions of Lactose
Reactions of Sucrose
Identification of Unknown Carbohydrate

3. Reactions of Lipids

Reactions of Triacylglycerols
Reactions of Cholesterol

4. Reactions of Proteins

Precipitation Reactions of Proteins
Colour Reactions of Proteins
Reactions of Albumin
Reactions of Casein
Reactions of Gelatin
Reactions of Peptone
Identification of Unknown Protein

5. Reactions of Non-protein Nitrogenous (NPN) Substances

Urea
Uric Acid
Creatinine
Identification of Unknown NPN Substance

6. Identification of Unknown Substance of Biochemical Importance
Carbohydrates, Proteins and NPN Substances

7. Analysis of Urine

Physical Examination
Microscopic Examination
Chemical Analysis

8. Analysis of Abnormal Constituents in Urine

Glucose
Ketone Bodies
Proteins
Blood
Bile Salts
Bile Pigments
Identification of Unknown Abnormal Constituents in Urine

9. Q
ualitative Analysis of Saliva

10. Qualitative Analysis of Gastric Juice


11. Qualitative Analysis of Bile


12. Qualitative Analysis of Milk


13. Food Ana
lysis

QUANTITATIVE EXPERIMENTS


14. Introduction to Clinical Biochemistry

Glassware and Instruments Used in the Laboratory
Solutions
Sample Collection and Handling
Colorimetry
ABG Analysis
Immunodiffusion
Quality Control

15.
Blood Glucose

16.
Glucose Tolerance Test

17. Blood Urea and BUN


18. Serum and Urinary Creatinine


19. Uric Acid


20. Serum Total Proteins and Albumin–Globulin Ratio


21. Cholesterol and Lipid Profile


22. Bilirubin


23. Aminotransferases (AST and ALT or SGOT and SGPT)


24. Alkaline and Acid Phosphatases (ALP and ACP)


25. Creatine Kinase and Its Isoenzymes


26. Amylase and Lipase


27. Calcium and Phosphorus


28. Serum Electrolytes


29. Quantitative Analysis of
CSF

30. Electrophoresis

Paper Electrophoresis
Gel Electrophoresis
Isoelectric Focussing
Capillary Electrophoresis

31. Chromatography

Thin Layer Chromatography (TLC)
Gas–Liquid Chromatography
Ion Exchange Chromatography
Gel Exclusion (Permeation) Chromatography
Affinity Chromatography
High Performance (Pressure) Liquid Chromatography (HPLC)

32. Spectroscopic Examination of Hemoglobin and Its Derivatives


33. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA)


34. Estimation of pH and Preparation of Buffers


35. Biomedical Waste Management


36. DNA Isolation and Quantification


37. Biochemica
l Tests for Common Clinical Conditions

APPENDICES

  1. Practical Biochemistry Question Bank
  2. Multiple-choice Questions (MCQs) in Practical Biochemistry
  3. III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry
  4. IV. Test Procedures for Qualitative and Quantitative Experiments in a Nutshell for Ready Reference

Index

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