Preface to the Second Edition 
Preface to the First Edition 
QUALITATIVE
EXPERIMENTS
1. Safety in the Laboratory 
2. Reactions of Carbohydrates 
Reactions of Glucose 
Reactions of Fructose 
Reactions of Maltose 
Reactions of Lactose 
Reactions of Sucrose 
Identification of Unknown Carbohydrate 
3. Reactions of Lipids 
Reactions of Triacylglycerols 
Reactions of Cholesterol 
4. Reactions of Proteins 
Precipitation Reactions of Proteins 
Colour Reactions of Proteins 
Reactions of Albumin 
Reactions of Casein 
Reactions of Gelatin 
Reactions of Peptone 
Identification of Unknown Protein 
5. Reactions of Non-protein Nitrogenous (NPN) Substances 
Urea 
Uric Acid 
Creatinine 
Identification of Unknown NPN Substance 
6. Identification of Unknown Substance of Biochemical Importance 
Carbohydrates, Proteins and NPN Substances 
7. Analysis of Urine 
Physical Examination 
Microscopic Examination 
Chemical Analysis 
8. Analysis of Abnormal Constituents in Urine 
Glucose 
Ketone Bodies 
Proteins 
Blood 
Bile Salts 
Bile Pigments 
Identification of Unknown Abnormal
Constituents in Urine 
9. Qualitative Analysis of Saliva 
10. Qualitative Analysis of Gastric Juice 
11. Qualitative Analysis of Bile 
12. Qualitative Analysis of Milk 
13. Food Analysis 
QUANTITATIVE
EXPERIMENTS 
14. Introduction to Clinical Biochemistry 
Glassware and Instruments Used in the
Laboratory 
Solutions 
Sample Collection and Handling 
Principles of Colorimetry 
Quality Control 
15. Blood Glucose 
16. Glucose Tolerance Test 
17. Blood Urea and BUN 
18. Serum and Urinary Creatinine 
19. Uric Acid 
20. Serum Total Proteins and Albumin–Globulin Ratio 
21. Cholesterol and Lipid Profile 
22. Bilirubin 
23. Aminotransferases (AST and ALT) 
24. Alkaline and Acid Phosphatases (ALP and ACP) 
25. Creatine Kinase and its Isoenzymes 
26. Amylase and Lipase 
27. Calcium and Phosphorus 
28. Serum Electrolytes 171 
29. Quantitative Analysis of CSF 
30. Electrophoresis 
31. Chromatography 
32. Spectroscopic Examination of Hemoglobin and its Derivatives 
33. Radioimmunoassay and Enzyme-linked Immunosorbant Assay