A Mapping of the Contents to CBME Practical Biochemistry Curriculum
Preface to the Fourth Edition
Preface to the First Edition

1. Safety in the Laboratory
Precautions
First Aid in the Laboratory

QUALITATIVE EXPERIMENTS
2. Reactions of Carbohydrates
Molisch’s Test
Benedict’s Test
Barfoed’s Test
Seliwanoff ’s Test
Foulger’s Test
Rapid Furfural Test
Osazone Test
Acid Hydrolysis
Methylamine Test for Lactose
Mucic Acid Test for Galactose
Bial’s Test for Pentoses
Iodine Test for Polysaccharides
Experiments
Reactions of glucose
Reactions of fructose
Reactions of maltose
Reactions of lactose
Reactions of sucrose
Identification of unknown carbohydrate
3. Reactions of Lipids
Reactions of Triacylglycerols
Reactions of Cholesterol
4. Reactions of Proteins
Precipitation Reactions of Proteins
Colour Reactions of Proteins
Experiments
Reactions of albumin
Reactions of casein
Reactions of gelatin
Reactions of peptone
Identification of unknown protein
5. Reactions of Non-protein Nitrogenous (NPN) Substances
Urea
Uric Acid
Creatinine
Identification of unknown NPN substance
6. Identification of Unknown Substance of Biochemical Importance
Carbohydrates, Proteins and NPN Substances
7. Analysis of Urine
Physical Examination
Microscopic Examination
Chemical Analysis
8. Analysis of Abnormal Constituents of Urine
Glucose
Ketone Bodies
Proteins
Blood
Bile Salts
Bile Pigments
Identification of unknown abnormal constituents in urine
9. Qualitative Analysis of Saliva
10. Qualitative Analysis of Gastric Juice
11. Qualitative Analysis of Bile
12. Qualitative Analysis of Milk
13. Food Analysis

QUANTITATIVE EXPERIMENTS
14. Clinical Biochemistry Laboratory: Instrumentation and Techniques
Glassware and Instruments Commonly Used in the Laboratory
Solutions
Sample Collection and Handling
Colorimetry
Spectrophotometer
Autoanalyser
Flame Photometer
Fluorimeter
Dry Chemistry Systems
ABG Analysis
Immunodiffusion
Quality Control
15. Blood Glucose
Sample Collection
Determination of Glucose in Body Fluids
16. Glucose Tolerance Test
Indications for GTT
Glucose Tolerance Curve
17. Blood Urea and BUN
Methods of Blood Urea Estimation
Urinary Urea and Urea Clearance
18. Serum and Urinary Creatinine and Creatinine Clearance
Creatine and Creatinine
Creatinine Clearance
19. Uric Acid
Formation and Excretion of Uric Acid
Methods of Estimation
20. Serum Total Proteins and Albumin–Globulin Ratio
21. Cholesterol and Lipid Profile
22. Bilirubin
Serum Bilirubin
23. Aminotransferases (AST and ALT or SGOT and SGPT)
24. Alkaline and Acid Phosphatases (ALP and ACP)
Estimation of Alkaline Phosphatase
Estimation of Acid Phosphatase
25. Creatine Kinase and Its Isoenzymes
25. Amylase and Lipase
Estimation of Serum Amylase
Estimation of Serum Lipase
26. Calcium and Phosphorus
26. Serum Electrolytes
Electrolyte Analysis by Ion Selective Electrode
27. Quantitative Analysis of CSF
27. Electrophoresis
Types of Electrophoresis
28. Chromatography
28. Spectroscopic Examination of Hemoglobin and Its Derivatives
29. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA)
Radio Immunoassay (RIA)
Enzyme-linked Immunosorbent Assay (ELISA)
30. Estimation of pH and Preparation of Buffers
Determination of pH Using a pH Meter
Buffers
31. Biomedical Waste Management
Segregation of Waste
Storage of Waste
Transportation of Waste
Treatment and Disposal of Waste
Safety Measures
32. DNA Isolation and Quantification
Basic Steps of DNA Isolation
Quantification of DNA
33. Biochemical Tests for Common Clinical Conditions
Diabetes Mellitus
Dyslipidemia
Myocardial Infarction
Renal Failure
Gout
Proteinuria
Nephrotic Syndrome
Edema
Jaundice
Liver Disorders
Pancreatitis
Acid-Base Disorders
Thyroid Disorders
Vitamins and Minerals
34. Urinary Screening for Inborn Errors of Metabolism
35. Energy Content of Various Foods and the Significance of Glycemic Index
Energy Requirements in Humans
Energy Content of Foods
Glycemic Index
36. Saturated, Unsaturated and Trans-Fatty Acids in Foods
Saturated Fatty Acids
Unsaturated Fatty Acids
Biomedical Importance of PUFA
Trans-fats
APPENDICES
I. Practical Biochemistry Question Bank
Qualitative Experiments
Quantitative Experiments
II. Multiple-choice Questions (MCQs) in Practical Biochemistry
III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry
IV. Test Procedures of Qualitative and Quantitative Experiments in a Nutshell
Qualitative Test Procedures (in alphabetical order)
Quantitative Test Procedures
Spotters and Charts

Index